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gmpcpp-stabilized microtubule seeds labeled with tamra dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gmpcpp-stabilized microtubule seeds labeled with tamra dye
    (A) Schematic of an IRM in vitro microtubule assay. Dynamic, label-free microtubules were grown from <t>TAMRA-labeled</t> <t>GMPCPP-stabilized</t> microtubule seeds attached to a silanized coverslip through anti-TAMRA antibodies. Microtubule dynamics was observed by time-lapse IR microscopy. Microtubule plus ends (marked with gray arrows) and minus ends were analyzed in this assay. Illumination light (blue line) is reflected from the glass/water interface and water/microtubule interface. Microtubule image is formed by the interference of reflected light. I IRM : interference intensity; I 0 : incident light intensity; I 1 : intensity of light reflected of glass/sample interface; I 2 : intensity of light reflected from water/microtubule interface (B) Representative kymographs depicting dynamic behavior of individual microtubule polymers in the presence or absence of NADs or NMN (as specified in the top right corner). Dashed lines indicate the position of TAMRA-labeled microtubule seeds. For all kymographs, microtubule plus-end is positioned to the right of the seed and corresponds to the orientation of microtubule on the illustration in panel (A) Horizontal scale bars, 3μm. Vertical scale bars, 5 minutes. (C) Scatter plots representing the effect of NADs and NMN on parameters of microtubule dynamic instability for an individual experimental repeat. Points on the diagrams depicting microtubule growth and shrinkage rates correspond to the average growth or shrinkage rate of individual microtubule within the sample. Time to catastrophe represents the lifetime of all analyzed events within the repeat. Red line indicates the average, blue error bars correspond to standard deviation (SD). The exact values for parameters of microtubule dynamic instability plotted here can be found in in bold. (D) Plots representing averages for all individual experiments. Same shape symbols correspond to the experiments performed side-by-side on the same day. Detail values for depicted averages are combined in . (E) Plots representing average growth rates and time to catastrophe measured for microtubule minus-ends. Averages for all individual experiments are combined in . Shaded boxes in panels D and E represents 95% confidence intervals with middle lines corresponding to the averages calculated based on the experimental repeats.
    Gmpcpp Stabilized Microtubule Seeds Labeled With Tamra Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gmpcpp-stabilized+microtubule+seeds+labeled+with+tamra+dye/gmpcpp+stabilized+microtubule+seeds+labeled+with+tamra+dye/pmc06687165-102-5-7
    Average 90 stars, based on 1 article reviews
    gmpcpp-stabilized microtubule seeds labeled with tamra dye - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Nicotinamide adenine dinucleotides and their precursor NMN have no direct effect on microtubule dynamics in purified brain tubulin"

    Article Title: Nicotinamide adenine dinucleotides and their precursor NMN have no direct effect on microtubule dynamics in purified brain tubulin

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0220794

    (A) Schematic of an IRM in vitro microtubule assay. Dynamic, label-free microtubules were grown from TAMRA-labeled GMPCPP-stabilized microtubule seeds attached to a silanized coverslip through anti-TAMRA antibodies. Microtubule dynamics was observed by time-lapse IR microscopy. Microtubule plus ends (marked with gray arrows) and minus ends were analyzed in this assay. Illumination light (blue line) is reflected from the glass/water interface and water/microtubule interface. Microtubule image is formed by the interference of reflected light. I IRM : interference intensity; I 0 : incident light intensity; I 1 : intensity of light reflected of glass/sample interface; I 2 : intensity of light reflected from water/microtubule interface (B) Representative kymographs depicting dynamic behavior of individual microtubule polymers in the presence or absence of NADs or NMN (as specified in the top right corner). Dashed lines indicate the position of TAMRA-labeled microtubule seeds. For all kymographs, microtubule plus-end is positioned to the right of the seed and corresponds to the orientation of microtubule on the illustration in panel (A) Horizontal scale bars, 3μm. Vertical scale bars, 5 minutes. (C) Scatter plots representing the effect of NADs and NMN on parameters of microtubule dynamic instability for an individual experimental repeat. Points on the diagrams depicting microtubule growth and shrinkage rates correspond to the average growth or shrinkage rate of individual microtubule within the sample. Time to catastrophe represents the lifetime of all analyzed events within the repeat. Red line indicates the average, blue error bars correspond to standard deviation (SD). The exact values for parameters of microtubule dynamic instability plotted here can be found in in bold. (D) Plots representing averages for all individual experiments. Same shape symbols correspond to the experiments performed side-by-side on the same day. Detail values for depicted averages are combined in . (E) Plots representing average growth rates and time to catastrophe measured for microtubule minus-ends. Averages for all individual experiments are combined in . Shaded boxes in panels D and E represents 95% confidence intervals with middle lines corresponding to the averages calculated based on the experimental repeats.
    Figure Legend Snippet: (A) Schematic of an IRM in vitro microtubule assay. Dynamic, label-free microtubules were grown from TAMRA-labeled GMPCPP-stabilized microtubule seeds attached to a silanized coverslip through anti-TAMRA antibodies. Microtubule dynamics was observed by time-lapse IR microscopy. Microtubule plus ends (marked with gray arrows) and minus ends were analyzed in this assay. Illumination light (blue line) is reflected from the glass/water interface and water/microtubule interface. Microtubule image is formed by the interference of reflected light. I IRM : interference intensity; I 0 : incident light intensity; I 1 : intensity of light reflected of glass/sample interface; I 2 : intensity of light reflected from water/microtubule interface (B) Representative kymographs depicting dynamic behavior of individual microtubule polymers in the presence or absence of NADs or NMN (as specified in the top right corner). Dashed lines indicate the position of TAMRA-labeled microtubule seeds. For all kymographs, microtubule plus-end is positioned to the right of the seed and corresponds to the orientation of microtubule on the illustration in panel (A) Horizontal scale bars, 3μm. Vertical scale bars, 5 minutes. (C) Scatter plots representing the effect of NADs and NMN on parameters of microtubule dynamic instability for an individual experimental repeat. Points on the diagrams depicting microtubule growth and shrinkage rates correspond to the average growth or shrinkage rate of individual microtubule within the sample. Time to catastrophe represents the lifetime of all analyzed events within the repeat. Red line indicates the average, blue error bars correspond to standard deviation (SD). The exact values for parameters of microtubule dynamic instability plotted here can be found in in bold. (D) Plots representing averages for all individual experiments. Same shape symbols correspond to the experiments performed side-by-side on the same day. Detail values for depicted averages are combined in . (E) Plots representing average growth rates and time to catastrophe measured for microtubule minus-ends. Averages for all individual experiments are combined in . Shaded boxes in panels D and E represents 95% confidence intervals with middle lines corresponding to the averages calculated based on the experimental repeats.

    Techniques Used: In Vitro, Labeling, Microscopy, Standard Deviation

    Related Articles

    Labeling:

    Article Title: Nicotinamide adenine dinucleotides and their precursor NMN have no direct effect on microtubule dynamics in purified brain tubulin
    Article Snippet: GMPCPP-stabilized microtubule seeds labeled with TAMRA dye (ThermoFisher Scientific, Waltham, MA) were used to initiate the growth of dynamic microtubule extensions from unlabeled GTP-tubulin.

    In Vitro:

    Article Title: Nicotinamide adenine dinucleotides and their precursor NMN have no direct effect on microtubule dynamics in purified brain tubulin
    Article Snippet: GMPCPP-stabilized microtubule seeds labeled with TAMRA dye (ThermoFisher Scientific, Waltham, MA) were used to initiate the growth of dynamic microtubule extensions from unlabeled GTP-tubulin.

    Microscopy:

    Article Title: Nicotinamide adenine dinucleotides and their precursor NMN have no direct effect on microtubule dynamics in purified brain tubulin
    Article Snippet: GMPCPP-stabilized microtubule seeds labeled with TAMRA dye (ThermoFisher Scientific, Waltham, MA) were used to initiate the growth of dynamic microtubule extensions from unlabeled GTP-tubulin.

    Standard Deviation:

    Article Title: Nicotinamide adenine dinucleotides and their precursor NMN have no direct effect on microtubule dynamics in purified brain tubulin
    Article Snippet: GMPCPP-stabilized microtubule seeds labeled with TAMRA dye (ThermoFisher Scientific, Waltham, MA) were used to initiate the growth of dynamic microtubule extensions from unlabeled GTP-tubulin.



    Similar Products

    gmpcpp-stabilized microtubule seeds labeled with tamra dye  (Thermo Fisher)
    90
    Thermo Fisher gmpcpp-stabilized microtubule seeds labeled with tamra dye
    (A) Schematic of an IRM in vitro microtubule assay. Dynamic, label-free microtubules were grown from <t>TAMRA-labeled</t> <t>GMPCPP-stabilized</t> microtubule seeds attached to a silanized coverslip through anti-TAMRA antibodies. Microtubule dynamics was observed by time-lapse IR microscopy. Microtubule plus ends (marked with gray arrows) and minus ends were analyzed in this assay. Illumination light (blue line) is reflected from the glass/water interface and water/microtubule interface. Microtubule image is formed by the interference of reflected light. I IRM : interference intensity; I 0 : incident light intensity; I 1 : intensity of light reflected of glass/sample interface; I 2 : intensity of light reflected from water/microtubule interface (B) Representative kymographs depicting dynamic behavior of individual microtubule polymers in the presence or absence of NADs or NMN (as specified in the top right corner). Dashed lines indicate the position of TAMRA-labeled microtubule seeds. For all kymographs, microtubule plus-end is positioned to the right of the seed and corresponds to the orientation of microtubule on the illustration in panel (A) Horizontal scale bars, 3μm. Vertical scale bars, 5 minutes. (C) Scatter plots representing the effect of NADs and NMN on parameters of microtubule dynamic instability for an individual experimental repeat. Points on the diagrams depicting microtubule growth and shrinkage rates correspond to the average growth or shrinkage rate of individual microtubule within the sample. Time to catastrophe represents the lifetime of all analyzed events within the repeat. Red line indicates the average, blue error bars correspond to standard deviation (SD). The exact values for parameters of microtubule dynamic instability plotted here can be found in in bold. (D) Plots representing averages for all individual experiments. Same shape symbols correspond to the experiments performed side-by-side on the same day. Detail values for depicted averages are combined in . (E) Plots representing average growth rates and time to catastrophe measured for microtubule minus-ends. Averages for all individual experiments are combined in . Shaded boxes in panels D and E represents 95% confidence intervals with middle lines corresponding to the averages calculated based on the experimental repeats.
    Gmpcpp Stabilized Microtubule Seeds Labeled With Tamra Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gmpcpp-stabilized+microtubule+seeds+labeled+with+tamra+dye/gmpcpp+stabilized+microtubule+seeds+labeled+with+tamra+dye/pmc06687165-102-5-7
    Average 90 stars, based on 1 article reviews
    gmpcpp-stabilized microtubule seeds labeled with tamra dye - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic of an IRM in vitro microtubule assay. Dynamic, label-free microtubules were grown from TAMRA-labeled GMPCPP-stabilized microtubule seeds attached to a silanized coverslip through anti-TAMRA antibodies. Microtubule dynamics was observed by time-lapse IR microscopy. Microtubule plus ends (marked with gray arrows) and minus ends were analyzed in this assay. Illumination light (blue line) is reflected from the glass/water interface and water/microtubule interface. Microtubule image is formed by the interference of reflected light. I IRM : interference intensity; I 0 : incident light intensity; I 1 : intensity of light reflected of glass/sample interface; I 2 : intensity of light reflected from water/microtubule interface (B) Representative kymographs depicting dynamic behavior of individual microtubule polymers in the presence or absence of NADs or NMN (as specified in the top right corner). Dashed lines indicate the position of TAMRA-labeled microtubule seeds. For all kymographs, microtubule plus-end is positioned to the right of the seed and corresponds to the orientation of microtubule on the illustration in panel (A) Horizontal scale bars, 3μm. Vertical scale bars, 5 minutes. (C) Scatter plots representing the effect of NADs and NMN on parameters of microtubule dynamic instability for an individual experimental repeat. Points on the diagrams depicting microtubule growth and shrinkage rates correspond to the average growth or shrinkage rate of individual microtubule within the sample. Time to catastrophe represents the lifetime of all analyzed events within the repeat. Red line indicates the average, blue error bars correspond to standard deviation (SD). The exact values for parameters of microtubule dynamic instability plotted here can be found in in bold. (D) Plots representing averages for all individual experiments. Same shape symbols correspond to the experiments performed side-by-side on the same day. Detail values for depicted averages are combined in . (E) Plots representing average growth rates and time to catastrophe measured for microtubule minus-ends. Averages for all individual experiments are combined in . Shaded boxes in panels D and E represents 95% confidence intervals with middle lines corresponding to the averages calculated based on the experimental repeats.

    Journal: PLoS ONE

    Article Title: Nicotinamide adenine dinucleotides and their precursor NMN have no direct effect on microtubule dynamics in purified brain tubulin

    doi: 10.1371/journal.pone.0220794

    Figure Lengend Snippet: (A) Schematic of an IRM in vitro microtubule assay. Dynamic, label-free microtubules were grown from TAMRA-labeled GMPCPP-stabilized microtubule seeds attached to a silanized coverslip through anti-TAMRA antibodies. Microtubule dynamics was observed by time-lapse IR microscopy. Microtubule plus ends (marked with gray arrows) and minus ends were analyzed in this assay. Illumination light (blue line) is reflected from the glass/water interface and water/microtubule interface. Microtubule image is formed by the interference of reflected light. I IRM : interference intensity; I 0 : incident light intensity; I 1 : intensity of light reflected of glass/sample interface; I 2 : intensity of light reflected from water/microtubule interface (B) Representative kymographs depicting dynamic behavior of individual microtubule polymers in the presence or absence of NADs or NMN (as specified in the top right corner). Dashed lines indicate the position of TAMRA-labeled microtubule seeds. For all kymographs, microtubule plus-end is positioned to the right of the seed and corresponds to the orientation of microtubule on the illustration in panel (A) Horizontal scale bars, 3μm. Vertical scale bars, 5 minutes. (C) Scatter plots representing the effect of NADs and NMN on parameters of microtubule dynamic instability for an individual experimental repeat. Points on the diagrams depicting microtubule growth and shrinkage rates correspond to the average growth or shrinkage rate of individual microtubule within the sample. Time to catastrophe represents the lifetime of all analyzed events within the repeat. Red line indicates the average, blue error bars correspond to standard deviation (SD). The exact values for parameters of microtubule dynamic instability plotted here can be found in in bold. (D) Plots representing averages for all individual experiments. Same shape symbols correspond to the experiments performed side-by-side on the same day. Detail values for depicted averages are combined in . (E) Plots representing average growth rates and time to catastrophe measured for microtubule minus-ends. Averages for all individual experiments are combined in . Shaded boxes in panels D and E represents 95% confidence intervals with middle lines corresponding to the averages calculated based on the experimental repeats.

    Article Snippet: GMPCPP-stabilized microtubule seeds labeled with TAMRA dye (ThermoFisher Scientific, Waltham, MA) were used to initiate the growth of dynamic microtubule extensions from unlabeled GTP-tubulin.

    Techniques: In Vitro, Labeling, Microscopy, Standard Deviation